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(26) showed that the mean QMVC in COPD patients declined by 4.3% over a year compared to 1-2% in a healthy aging population (27)

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Serial 10-fold dilutions of homogenates were plated on specific media for Lactobacillus (MRS media, Oxoid) or Bifidobacterium (MRS media supplemented with dicloxacilin (0·5 mg/l), LiCl (1 g/l) and l-cysteine hydrochloride (0·5 g/l)) and incubated under anaerobic conditions in an anaerobic chamber for 24–48 h at 37°C